cell type  (Multi Sciences (Lianke) Biotech Co Ltd)

Journal: International Journal of Molecular Medicine

Article Title: Ebastine targets HER2/HER3 signaling and cancer stem cell traits to overcome trastuzumab resistance in HER2-positive breast cancer

doi: 10.3892/ijmm.2026.5751

Figure Lengend Snippet: EBA downregulates HER2, p95HER2, HER3 and AKT expression. (A) Immunoblot analysis of HER2, p95HER2 and p-HER2 (Y1221/1222) in JIMT-1 cells treated with EBA for 48 h. (B) Immunoblot analysis of HER3, p-HER3 (Y1289), AKT and p-AKT following treatment with EBA (48 h) in JIMT-1 cells. (C) Immunoblot analysis of HER2, HER3 and EGFR following IP with anti-HER2 antibody in JIMT-1 cells treated with EBA. In silico molecular docking of EBA with the crystal structure of HER2-KD. (D) Surface map of lipophilic and hydrophilic properties at the ATP-binding site of HER2-KD (red, hydrophobic; blue, hydrophilic). (E) 2D interaction diagram showing intermolecular interactions between EBA and HER2-KD. Key amino acid residues within the binding pocket are shown. (F) Predicted binding pose of EBA (purple stick model) within the tyrosine kinase domain of HER2 (blue ribbon). (G) 293T cells were treated with DMSO or EBA for 1 h at 37°C, followed by heating for 3 min. Soluble fractions were collected following centrifugation and analyzed by immunoblotting using an anti-HER2 antibody. EBA, ebastine; p-, phosphorylated; IP, immunoprecipitation; IB, immunoblotting; KD, kinase domain PCB, protein complex binding; TM, transmembrane; a.a., amino acid.

Article Snippet: 293T (American Type Culture Collection) cells were cultured overnight at 37°C in a humidified atmosphere with 5% CO 2 , and treated with either DMSO (vehicle) or 30 μ M ebastine for 1 h at 37°C.

Techniques: Expressing, Western Blot, In Silico, Binding Assay, Centrifugation, Immunoprecipitation

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: Dose-dependent effect of Mn-rods on the cell viability of ( A ) A549 and ( B ) Calu-3 cells after 24 and 48 h incubation at 37 °C. Cell viability was assessed using Alamar Blue metabolic assay and expressed as percentage relative to untreated cells (normalized to 100%). Data are shown as means ± SD. n=3. The statistical analysis was conducted using two-way ANOVA, p<0.05 (*), p<0.01 (**) and p<0.001 (***) compared to negative control (untreated cells) for each time point.

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Incubation, Metabolic Assay, Negative Control

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: ( A ) Cell viability of A549 cells after 24 and 48 h of incubation with Mn-rods (at 10, 20 and 30 µg/mL), assessed by Trypan Blue exclusion assay. Triton X 0.2% used as positive control. Data are shown as means ± SD. n=3. The statistical analysis was conducted using two-way ANOVA, p<0.01 (**) and p<0.001 (***) compared to negative control (untreated A549 cells) for each time point. ( B–F ) correspond to phase-contrast microscopy images of A549 after 48 h of exposure to Mn-rods ( B – D) – A549 cells treated with 10, 20 and 30 µg/mL of Mn-rods, respectively; ( E ) negative control (untreated A549 cells); ( F ) A549 cells incubated with Triton X 0.2%). The scale bar is 100 µm.

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Incubation, Trypan Blue Exclusion Assay, Positive Control, Negative Control, Microscopy

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: TEM images showing the cellular uptake of Mn-rods (10 µg/mL) by A549 and Calu-3 cells. Panels ( A – C ) correspond to A549 cells: ( A and B ) after 24 and 48 h of exposure to Mn-rods, respectively. ( C ) corresponds to untreated A549 cells. Panels ( D – F ) correspond to Calu-3 cells: ( D and E ) show Mn-rods internalization after 24 and 48 h of exposure, respectively. ( F ) corresponds to untreated Calu-3 cells. Yellow dashed circles highlight internalized Mn-rods, and yellow dashed lines mark the areas shown at higher magnification.

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: Relative intracellular ROS levels in A549 cells, measured by DCF fluorescence after 4 h of incubation with Mn-rods (10 µg/mL). Tert-butyl hydroperoxide (TBHP, 250 µM) was used as the positive control. Data are shown as means ± SD. n=3. The statistical analysis was conducted using one-way ANOVA, p<0.001 (***) compared to negative control (untreated A549 cells).

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Fluorescence, Incubation, Positive Control, Negative Control

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: Identification of the highest non-toxic concentration of compounds targeting different signaling pathways in A549 cell line, using the Alamar Blue assay. Cells were incubated with the pan-caspase inhibitor zVAD-fmk, cathepsin B inhibitor CA-074, the receptor-interacting serine/threonine-protein kinase 1 (RIPK1) inhibitor necrostatin-1, the radical trapping antioxidant ferrostatin-1, the lipid reactive oxygen species scavenger liproxstatin-1, or the iron-chelating agent deferoxamine, and with the ferroptosis inducer, RSL-3, for 48 h at 37 °C. Data are shown as means ± SD. n=3. The statistical analysis was conducted using ordinary one-way ANOVA, ns – not significant, p<0.05 (*), p<0.01 (**) and p<0.001 (***). NC- negative control (untreated A549 cells).

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Concentration Assay, Protein-Protein interactions, Alamar Blue Assay, Incubation, Negative Control

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: Effect of RSL-3 (2 µM) and different inhibitors (the radical trapping antioxidant ferrostatin-1, 10 µM, the lipid reactive oxygen species scavenger liproxstatin-1, 10 µM, and the iron-chelating agent deferoxamine, 3 µM, on cell viability of A549 cells, following incubation for 48 h at 37 °C. Alamar Blue assay, data are shown as means ± SD. n=3. The statistical analysis was conducted using ordinary one-way ANOVA, p<0.01 (**) and p<0.001 (***). Negative control- negative control (untreated A549 cells).

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Incubation, Alamar Blue Assay, Negative Control

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: Study of the potential mechanisms underlying the effect on the metabolic activity of A549 cells after exposure for 48 h to Mn-rods in the presence or absence of the specified inhibitors (the pan-caspase inhibitor zVAD-fmk (5 µM), cathepsin B inhibitor CA-074 (10 µM), the receptor-interacting serine/threonine-protein kinase 1 (RIPK1) inhibitor necrostatin-1 (20 µM), the radical trapping antioxidant ferrostatin-1 (10 µM), the lipid reactive oxygen species scavenger liproxstatin-1 (10 µM), the iron-chelating agent deferoxamine (3 µM). RSL-3 (2 µM), ferroptosis inducer, was used as a positive control of ferroptosis. Data are shown as means ± SD. n=3. The statistical analysis was conducted using ordinary one-way ANOVA, ns – not significant, p<0.01 (**) and p<0.001 (***). Negative control- negative control (untreated A549 cells).

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Activity Assay, Positive Control, Negative Control